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. Author manuscript; available in PMC: 2020 Sep 8.
Published in final edited form as: Curr Protoc Neurosci. 2019 Feb 21;87(1):e63. doi: 10.1002/cpns.63

Figure 4.

Figure 4.

Example for RNAscope on fresh-frozen section (Basic Protocol 1). Expression of ErbB4 (magenta; Channel 1) was analyzed in excitatory neurons labeled by vesicular glutamate transporter 1 (vGLUT1; green; Channel 2) and in GABAergic neurons labeled by glutamate decarboxylase 1 (GAD1; white; Channel 3). (A) Overview of an adult coronal wildtype (WT; C57BL/6J) mouse brain section. (B) Magnification in the primary somatosensory cortex. Scale bars 500μm in A, 200μm in B. See figure 5 for analysis.