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. Author manuscript; available in PMC: 2020 Sep 8.
Published in final edited form as: Curr Protoc Neurosci. 2019 Feb 21;87(1):e63. doi: 10.1002/cpns.63

Figure 6.

Figure 6.

Example for RNAscope on FFPE sections (Alternate Protocol 1) and adherent cell culture (Alternate Protocol 2). (A) Expression of ErbB4 (C1, cyan) and Nkx2.1 (magenta, C3) on a coronal paraffin section of an E14.5 WT embryo. (A’) Magnification at the border between the medial and lateral ganglionic eminence. (B) Expression of ErbB4 (cyan, C1) in primary cultured dopaminergic neurons (prepared as in (Skirzewski et al., 2017)) identified by tyrosine hydroxylase (Th, C3, magenta) at DIV13. Scale bars 500μm in A and 50μm in A’, 20μm in B.