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. 2020 Jul 29;42:101055. doi: 10.1016/j.molmet.2020.101055

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Ablation of Aster-B or inhibition of Arf1 causes mitochondrial dysfunction. (A) Seahorse XF analysis showing oxygen consumption rate (OCR) of C2C12 vector control with vehicle (VC) or 10 μM of Exo-2 (VC Exo-2) and Aster-B knockout (KO) cells treated with MCD for 2 hr and then treated with 25 μM non-fluorescent cholesterol ester for 1 h. Cells were treated with vehicle or 10 μM of Exo-2 throughout the assay. (B) Basal, (C) maximum, (D) ATP-linked respiration of the Seahorse XF analysis is shown. n = 5. ∗∗p < 0.01, ∗∗∗∗p < 0.0001 by student's t-test.