Induction of osteoporosis |
No ovariectomy |
Double ovariectomy was done in the beginning of the experiment |
Subcutaneous TPTD injection (12th week) |
No injection |
Subcutaneous injections 6 days a week |
No injection |
LLLT application (12th week) |
No application |
LLLT application on the lower jaw and left femur every 48 hours (3 times a week). |
Euthanization dates &dissection of animals |
One third of each group were euthanized at the end of weeks: 16, 19 and 24. |
Lower jaws (divided into right and left halves) and left femurs were dissected. |
Bone density measurement |
Done using CT at the end of weeks: 16 and 24. Assessment was done on lower jaws and left femurs. |
Histopathological examination |
Done using ordinary light microscope at the end of weeks: 16, 19 and 24. Assessment was done on right halves of jaws and femur shafts. |
Measurement of average size of bone pores |
Done using SEM at the end of weeks: 16, 19 and 24. Assessment was done on left halves of jaws. |
Calcium & phosphorus elemental analysis |
Done using EDAX at the end of weeks: 16, 19 and 24. Assessment was done on left halves of jaws. |
Measurement of PINP levels |
Done using an ELISA kit at the end of weeks: 16, 19 and 24. Assessment was done on femur head and neck. |