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. 2020 Aug 1;13(8):1970–1985.

Table 1.

Experimental procedure

Procedure Groups

NC OC T T+L L
Induction of osteoporosis No ovariectomy Double ovariectomy was done in the beginning of the experiment
Subcutaneous TPTD injection (12th week) No injection Subcutaneous injections 6 days a week No injection
LLLT application (12th week) No application LLLT application on the lower jaw and left femur every 48 hours (3 times a week).
Euthanization dates &dissection of animals One third of each group were euthanized at the end of weeks: 16, 19 and 24.
Lower jaws (divided into right and left halves) and left femurs were dissected.
Bone density measurement Done using CT at the end of weeks: 16 and 24. Assessment was done on lower jaws and left femurs.
Histopathological examination Done using ordinary light microscope at the end of weeks: 16, 19 and 24. Assessment was done on right halves of jaws and femur shafts.
Measurement of average size of bone pores Done using SEM at the end of weeks: 16, 19 and 24. Assessment was done on left halves of jaws.
Calcium & phosphorus elemental analysis Done using EDAX at the end of weeks: 16, 19 and 24. Assessment was done on left halves of jaws.
Measurement of PINP levels Done using an ELISA kit at the end of weeks: 16, 19 and 24. Assessment was done on femur head and neck.