Fig. 1.

Structural changes in airways from CFTR−/− piglets. a Comparison of cross-sections of tracheas from CFTR+/+ (n = 2) and CFTR−/− (n = 2) newborn piglets. Bars = 500 μM. b Higher magnification showing defective cartilage structure in muscle bundles in CFTR−/− tracheas. Bars = 80 μM. c Small airways from CFTR+/+ (n = 3) and CFTR−/− (n = 3) newborn piglets. Dark precipitation is caused by 4-hydroxy-2-nonenal (4-HNE) staining, indicating membrane lipid peroxidation of airway epithelial cells in CFTR−/− tracheas. Typically, 34–40 images were examined in each series. Bars = 50 μM