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. 2020 Jul 25;472(10):1507–1519. doi: 10.1007/s00424-020-02440-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Lack of intestinal fluid and mucus transport in CFTR−/− piglets. a Histology and Alcian blue staining of intestinal epithelium from CFTR+/+ and CFTR−/− piglets. Bars = 100 μM. Representative stainings from n = 2 CFTR+/+ and CFTR−/−, respectively, with 22–23 images being analyzed. b Expression of TMEM16A, TMEM16F, and CFTR in intestinal epithelial cells from CFTR+/+ and CFTR−/− piglets as detected by RT-PCR. c Original Ussing chamber recordings of transepithelial voltages (open circuit) from freshly isolated jejunal epithelium obtained from CFTR+/+ (upper recoding; black) and CFTR−/− (lower recording; red) piglets. d Comparison of calculated short circuit currents activated by basolateral IBMX (100 μM) and forskolin (2 μM) (IF) or the muscarinic agonist carbachol (CCH, 100 μM). e Scheme for the perfusion of isolated CFTR+/+ and CFTR−/− jejunum ex vivo (left) and f time course for mucus release induced by luminal perfusion with PGE₂ (1 μM) and ATP (100 μM). Mean ± SEM (number of animals/number of experiments or reactions). ^#Significant difference when compared with CFTR+/+ (p < 0.05, ANOVA)