Fig. 5.

Comparison of ion transport in airway epithelial cells from CFTR+/+ and CFTR−/− piglets. a Open circuit Ussing chamber measurements on polarized grown airway epithelial cells from CFTR+/+ and CFTR−/− piglets in the absence of CO₂/HCO₃⁻. Effects of amiloride (10 μM). b Comparison of voltage deflections and calculated equivalent short currents (Isc). c,d Effect of IBMX/forskolin (IF 100/2 μM) and CFTRinh172 (30 μM) on transepithelial voltages and Isc. e,f Effect of IF and bumetanide (100 μM) on transepithelial voltages and Isc. g,h Effect of luminal ATP (100 μM) on transepithelial voltages and Isc (pk, peak current; pl, plateau current). i,j Effect of luminal ATP (100 μM) in the presence of niflumic acid (NFA; 10 μM) on transepithelial voltages and Isc. Mean ± SEM (number of animals/number of measurements). *Significant effects of IF, Bum, or ATP (p < 0.05, paired t-test). ^#Significant difference when compared with CFTR+/+ (p < 0.05, unpaired t-test). ^$Significant difference when compared with the absence of NFA (p < 0.05, unpaired t-test)