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. 2020 Aug 16;472(10):1469–1480. doi: 10.1007/s00424-020-02443-9

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© The Author(s) 2020

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Fig. 7 — Expression of cholinergic receptors in the presence of a NO donor and a muscarinic receptor antagonist. Expression of M₃ and M₅ receptors was reduced significantly in response to SMN. a SNAP (200 μM) alone increased the expression of M₃ receptors, significantly. b SNAP alone (50, 100, and 200 μM) increased the expression of M₅ receptors, significantly. SMN along with SNAP decreased the stimulatory effects of SNAP on the expression of the two receptors (n = 3, **P < 0.01, ***P < 0.001, ****P < 0.0001). All values are expressed as ± SE. Abbreviations: M₃, muscarinic acetylcholine receptor 3; M₅, muscarinic acetylcholine receptor 5; NS, not significant; SMN, scopolamine methyl nitrate; SNAP, S-nitroso-N-acetylpenicillamine