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Rat primers used for quantitative real-time polymerase chain reaction
| Gene | Forward primer (5′–3′) | Annealing temp. (°C) | Reverse primer (3′–5′) | Annealing temp. (°C) | Product size (bp) |
| HPRT | GCAGACTTTGCTTTCCTTGG | 53.4 | TCCACTTTCGCTGATGACAC | 53.4 | 157 |
| NOS3 | GATGGCGAAGCGTGTGAAGG | 57.5 | GGCCTCATGCTCTAGGGATACC | 60.9 | 208 |
| M3 | TGCGCAGACAAGACCACGGC | 62 | GCGTCTGGGCGGCCTTCTTC | 62 | 142 |
| M5 | TTGCAGTTGTGACTGCGGTG | 56 | GAGAACCCAGCGTCCCATGA | 57 | 197 |
