FIGURE 3.

Validation of 2-MBT activity. (A) The chemical structure of 2-mercaptobenzothiazole (abbreviated as 2-MBT) was drawn using ACD/ChemSketch (ACD/ChemSketch, 2020). (B,C) Bacteria were grown in liquid media in the absence of NO with different concentrations of 2-MBT. Growth (B) and culturability (C) were monitored hourly. Solid points represent the mean of at least 3 independent replicates and the error bars reflect the standard error of the means. (D) [NO] following compound and DPTA addition at time = 0 was continuously measured using a NO probe. Quickly after the addition of DPTA, [NO] peaked at ∼3 μM. The time of NO clearance was identified as when [NO] ≤ 0.2 μM, which corresponded to ∼0.95 h for cells treated with DMSO. In Δhmp, the dominant NO detoxification enzyme under oxygenated conditions is absent, and therefore, the gray line corresponds to negligible cellular detoxification of NO. Solid lines are the average measurements of at least 3 biological replicates, and the shaded regions around the lines are the standard errors of the means.