FIGURE 9.
Protein output from PT5 in the presence and absence of NO with and without inhibitors. gfpSF was under the regulation of an IPTG-inducible PT5 promoter on pUA66. At time = 0, 2 mM IPTG were added to allow the full induction of expression, and protein abundance in the absence of NO (A) or in its presence (B) was measured by fluorescence via flow cytometry. Solid bars are the mean of the median fluorescence of 100,000 cellular events from 3 biological replicates, and the error bars the standard errors of the means. Asterisk signs (*) indicate when the fluorescence between compared groups were significantly different. Statistical significance was determined by first running a one-way ANOVA test across all treatment conditions at the same time point. When the p-value from the ANOVA test was lower than 0.05, difference between groups were assessed by performing Tukey’s test, with a p-value threshold of 0.05.