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. 2020 Aug 25;11:1080. doi: 10.3389/fphys.2020.01080

FIGURE 2.

FIGURE 2

BDNF assessment protocol at both pre- and posttest. Both pre- and posttest sessions started with 15 min of seated rest (Rest). Thereafter participants performed a training session of either: cognitive exercise only (CE) followed by 35 min of rest, physical exercise only (PE) followed by 35 min of seated rest, cognitive exercise followed by physical exercise (CE + PE) or physical exercise followed by cognitive exercise (PE + CE), according to their allocated intervention. Blood samples were drawn immediately after 15 min of rest (S1 and S4 at pretest and posttest, respectively), immediately after the first exercise (S2 and S5 at pretest and posttest, respectively) and immediately after the second exercise or rest (S3 and S6 at pretest and posttest, respectively).