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. 2020 Sep 7;6:42. doi: 10.1038/s41523-020-00183-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Time-lapse on Cytation of mobile osteoblasts contacting and drawing out cellular protrusion from MCF-7 (red). Scale bar is 100 µm. b Time-lapse on Incucyte S3 of osteoblast (unlabeled) drawing out cellular tether protrusion that leads to MCF-7 (RFP-labeled,) migration. Scale bar is 100 µm. c Deconvolution imaging of MCF-7 cell (green) with dendritic spine-like structures (DSLS) protrusions attached to OBs (red). Scale bar is 10 µm. d Tomogram of the terminus of DSLS (green) touching OB cell membrane (yellow). Detail of actin and ribosome concentration where cells make contact. Scale bar is 500 nm. e Tomogram of multiple DSLS structures and actin within. Scale bar is 500 nm. f The relative abundance of MCF-7 DSLS structures in co-culture with various bone cells, normalized to abundance in monoculture. OB: osteoblasts represented by hFOB 1.19 cells. PreOB: Pre-osteoblasts represented by MC3T3-E1 cells. OC: osteoclasts represented by RAW 264.7 cells. MSC: immortalized Mesenchymal Stem cells Fibro: fibroblasts represented by 293T cells. Error bars indicate standard deviation calculated based on binomial distribution (see “Methods”).