Table 2. Primers for PCR amplification and restriction enzyme information for genotyping of the MC1R gene in Asian ducks.
| Primer no. | Primer sequences (5′ to 3′) | Primer size (bp) | Location | Annealing Temp (°C) | Use | Restriction enzyme |
|---|---|---|---|---|---|---|
| P1 | F: CCATGTCCCCTTGACCTCG R: CCACTGCAAAGAGCCTTTATTCG |
1221 | −141 to +1080 | 63 | Direct sequencing | |
| P2 | F: GCTGAGGTCGGGGCCATGT R: CCGTGGCGTTGCTCTGGTTG |
91 | −15 to +76 | 63 | c.52A>G Genotyping |
MnlI |
| P3 | F: GCTCTTCATGCTGCTGATGG R: GGCAGGTGACGATGAGGATG |
515 | +278 to +793 | 63 | c.376A>G, c.409A>G, c.649C>T, Genotyping |
Hpy991 BclI BspMI |