Skip to main content
. 2016 Jul 25;53(3):223–232. doi: 10.2141/jpsa.0150162

Fig. 4.

Fig. 4.

Plasma membrane integrity of drake sperm as detected by immunofluorescence. SYBR-14: fluorescence staining with SYBR-14; PI: fluorescence staining with PI; DIC: differential interference contrast image; and Merge: overlap of dual fluorescence and DIC. Images were acquired using an Olympus IX81/FV1000 laser scanning confocal microscope (Olympus, Tokyo, Japan). Scale bar=20 µm.