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Mitochondrial function of drake sperm as assessed by immunofluorescence. R123: fluorescence staining with R123; PI: fluorescence staining with PI; DIC: differential interference contrast image; and Merge: overlap of dual fluorescence and DIC. Images were acquired using an Olympus IX81/FV1000 laser scanning confocal microscope (Olympus, Tokyo, Japan). Scale bar=20 µm.
