Fig. 4.

Immunohistochemical staining with the EM48 antibody showed aggregation of the hTT protein in the striatum of the hD rats. A and B : Formation of mutant hTT detected by the EM48 antibody at 11 weeks after AAV2 injection into the contralateral and ipsilateral striatum of the hD rats. Arrows indicate intranuclear aggregates, and arrowheads indicate smaller neuropil aggregates outside the nucleus. C and D : The number of EM48 aggregated cells counted was significantly different between the hD ×1 and hD ×10 subgroups in unilateral ipsilateral striatum (C) and bilateral striata (D) (one-way ANOVA with Turkey’s post-hoc test). *p<0.05. hTT : huntingtin gene, hD : huntington’s disease, AAV2 : adeno-associated viral vector, ANOVA : analysis of variance.