Table 5. Antibacterial effects of the purified OTFB and OTFC variants against S. aureus and E. coli.
| Time |
S. aureus |
E. coli |
||||
|---|---|---|---|---|---|---|
| Control1 | OTFB | OTFC | Control1 | OTFB | OTFC | |
| 0 h | 0.000±0.000 | 0.000±0.000 | 0.000±0.000 | 0.000±0.000 | 0.000±0.000 | 0.000±0.000 |
| 2 h | 0.032±0.000a | 0.008±0.002b | 0.011±0.003b | 0.018±0.009a | 0.003±0.001b | 0.008±0.002a |
| 4 h | 0.029±0.002a | 0.022±0.001b | 0.021±0.001b | 0.023±0.000a | 0.015±0.001b | 0.017±0.001b |
| 6 h | 0.060±0.000a | 0.051±0.005a | 0.051±0.004a | 0.044±0.002a | 0.035±0.003a | 0.034±0.003a |
| 8 h | 0.108±0.000a | 0.088±0.005b | 0.090±0.002a | 0.080±0.001a | 0.064±0.002b | 0.065±0.002b |
| 10 h | 0.130±0.002a | 0.107±0.005b | 0.111±0.002b | 0.086±0.002a | 0.070±0.000b | 0.073±0.001b |
| 12 h | 0.140±0.001a | 0.119±0.004b | 0.120±0.002b | 0.099±0.001a | 0.079±0.001b | 0.082±0.002b |
| 16 h | 0.185±0.001a | 0.139±0.005b | 0.142±0.002b | 0.131±0.006a | 0.098±0.004b | 0.096±0.002b |
Means (±SE) within a time and bacteria not sharing a common superscript differ (P<0.05). Values indicate the absorbance (A620nm) of the bacterial culture at the given time.
Control was incubated with PBS instead of the purified ovotransferrin.