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. 2020 Sep 7;10:14685. doi: 10.1038/s41598-020-71661-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression characteristics of OsF₃H and related genes, and LCMS–MS analysis of flavonol and anthocyanin profiling in response to WBPH stress. Bars represent mean ± standard deviation, asterisks indicate significant difference (p ˂ 0.05 two-way ANOVA, Bonferroni post-test) and ns indicate non-significant between wild and transgenic lines. 2 h, 12 h and 24 h represent data taking time points in hours while OxF₃H indicate transgenic line. (A) Expression level of OsF3H gene in transgenic line compare to wild type after WBPH infestation. (B–E) Effect of WBPH induced stress on expression of OsFLS, OsDFR, OsWRKY13 and OsSLR1 genes respectively in transgenic plants as compare to wild type. (F) LCMS–MS profiling of flavonol (kaempferol, quercetin) and anthocyanin (delphinidin, cyanidin). WC, TC, WT and TT indicate wild control, transgenic control, wild treated and transgenic treated with WBPH respectively. Kaempferol and cyanidin in wild control and wild treated plants.