Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 7;10:14683. doi: 10.1038/s41598-020-71645-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

TRPV6 controls levels of EMT-associated factors. (a) Expression of TRPV6 in different cell lines was analyzed by Western blotting from cellular lysates, with specific antibodies. E-cadherin and vimentin were used to assess epithelial/mesenchymal-like phenotype of the cells and GAPDH was used as a loading ctrl. Weight markers (kDa) for the indicated proteins are shown next to the blots. See also Supplementary Fig. 7a for uncut blots. (b) Quantifications of the protein levels, related to (a). Levels of TRPV6, E-cadherin and vimentin were assessed from the Western blot experiments of MCF10A, MCF7 and MDA-MB-231 cell lines. Both normal mammary epithelial cells and non-invasive MCF7 mammary epithelial cells displayed low TRPV6 expression together with low vimentin and high E-cadherin expression, while invasive MDA-MB231 cells displayed totally opposite expression pattern. Mean (± SEM) is shown. n = 3. *P < 0.05; **P < 0.01 (Paired t-test). Note that although MDA-MB-231 cells displayed much higher levels of TRPV6 and vimentin, due to the variation in between individual Western blot experiments, the statistical difference in between MDA-MB-231 cells and MCF10A or MCF7 cells was not significant (Paired t-test). (c) Western blotting was applied to analyze the expression of EMT-associated proteins from ctrl and TRPV6-depleted 184A1 cells. Specific antibodies against vimentin, snail, slug, N-cadherin and β-catenin were utilized. GAPDH acts as a loading control. Weight markers (kDa) for the indicated proteins are shown next to the blots. See also Supplementary Fig. 7b,c for uncut blots. (d) Quantification of vimentin and slug levels, related to (c). Intensity values were measured in ImageJ and intensity values for vimentin and slug were divided with the corresponding GAPDH values. Control sample values were normalized to 1. Mean (± SEM) is shown. n = 3. *P < 0.05 (Paired t-test). (e) Quantification of vimentin and slug levels upon TRPV6 overexpression, related to Supplementary Fig. S7d. Intensity values were measured in ImageJ and intensity values for vimentin and slug were divided with the corresponding GAPDH values. Control sample values were normalized to 1. Mean (± SEM) is shown. n = 3. *P < 0.05 (Paired t-test).