Table 3. Primers and probes used in DNA intestinal flora determination.

Target bacteria	Primers/Probes	Sequence (5′-3′)
Total bacteria	F-tot	GCAGGCCTAACACATGCAAGTC
	R-tot	CTGCTGCCTCCCGTAGGAGT
	Tot bac probe	CGTATTACCGCGGCTGCTGGCAC
Lactobacilli	F-lac	GCAGCAGTAGGGAATCTTCCA
	R-lac	GCATTYCACCGCTACACATG
	Lactob probe	CTGATGGAGCAACGCCGCGTG
Enterobacteria	F-ent	ATGGCTGTCGTCAGCTCGT
	R-ent	CCTACTTCTTTTGCAACCCACTC
	Enterob probe	CGTAGTCCGGATTGGAGTCTGCA
Clostridium perfrigens	ClosFor	AAGGCGACTCTCTGGACTGT
	ClosRev	CCCGAAGGGATTTCCTCGATTA
	Clos probe	CCCGCACAAGTAGCGGAGCA