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. 2020 Sep 7;10:14686. doi: 10.1038/s41598-020-71592-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NMDS plots of the urine metabolome. The centroid of each group is noted as a diamond. (a) NMDS plot generated with all samples included; (b) NMDS plot generated with only male samples; (c) NMDS plot generated with only female samples. Samples collected from mice at 5, 10, and 15 months are indicated in red, green and blue respectively; those collected from female and male mice are expressed with circles and triangles respectively; those collected from mice on the CD or WD are designated as open or closed markers respectively.