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. 2020 Sep 7;11(9):723. doi: 10.1038/s41419-020-02810-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Subcellular fractionation and FISH (scar bar, 10μm) were conducted to ascertain subcellular localization of LINC01559. c RT-qPCR followed by RIP assays demonstrated the enrichment of LINC01559, miR-6783-3p and miR-1343-3p in Ago2 or IgG group in indicated GC cells. GAPDH served as the normalized control of LINC01559 and U6 acted as that of miR-6783-3p and miR-1343-3p. Student’s T-test. d The binding sequences between LINC01559 and miR-1343-3p predicted by starBase. e, f RNA pull down (one-way ANOVA) and luciferase reporter assays (Student’s T-test) validated the relationship between LINC01559 and miR-1343-3p. U6 acted as the normalized control of miR-1343-3p enrichment in groups in RNA pull down assay. g Venn diagram showed the predicted mRNAs by three diagrams in starBase. h The binding sites between miR-1343-3p and PGK1 were predicted by starBase. i Luciferase reporter assay validated the relationship between miR-1343-3p and PGK1. Student’s T-test. j PGK1 protein expression was evaluated by western blot. **P < 0.01. “n.s.” indicates no significance.