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. 2020 Aug 25;7:216. doi: 10.3389/fmolb.2020.00216

FIGURE 4.

FIGURE 4

Circ_0005567 derepressed ATG14 expression by sponging miR-495. (A) RNA pull-down was performed to analyze the interaction between circ_0005567 and miR-495. (B) Sequences of the putative miR-495 binding sites in the 3′- UTR of ATG14. Luciferase activity in chondrocytes co-transfected with wild type (WT) or mutant (Mut) ATG14 3′-UTR luciferase reporter plasmids and miR-495 mimics or mimic NC. (C) MiR-495 expression, (D) circ_0005567 expression, (E) ATG14 mRNA level, and (F) ATG14 protein level in chondrocytes transfected with miR-495 mimic, mimic NC, miR-495 inhibitor, and inhibitor NC. (G) ATG14 mRNA level and (H) ATG14 protein level in chondrocytes co-transfected with miR-495 mimic/mimic NC and circ_0005567 overexpression vector/empty vector. The data are expressed as mean ± standard deviation from three independent experiments. *P < 0.05, **P < 0.01, versus the NC or mimic NC or mimic NC + Vector group; ##P < 0.01, versus the inhibitor NC or mimic NC + circ_0005567 or Vector + miR-495 mimic group.