TABLE 4.

Drosophila peroxisome matrix enzymes without a PTS1 motif.

Human	Drosophila	Function	Function	Loc
ACAA1	CG9149	β-Oxidation	β-Ketoacyl-CoA thiolase	(−)B
ACAD11	CG4860	β-Oxidation	Short-chain acyl-CoA dehydrogenase	(−)B
ACOX2	CG17544	β-Oxidation	Acyl-CoA oxidase	(+)B
ACSL1	CG3961	β-Oxidation	Long-chain-fatty-acid–CoA ligase	(−)B
ACSL3/4	Acsl	β-Oxidation	Acyl-CoA synthetase long-chain	(−)B (+)H
DECR2	CG3699	β-Oxidation	17-Beta-estradiol 17-dehydrogenase	(−)B
DRS7B	CG31548	β-Oxidation	17-Beta-estradiol 17-dehydrogenase	(−)B
EPHX2	Pummelig	ROS metabolism	Carboxylesterase	(−)B (+)HT
HMGCL	Hmgcl	Amino acid metabolism	Hydroxymethylglutaryl-CoA lyase	(+)B
HMGCR	Hmgcr	Isoprenoid synthesis	HMG coenzyme A reductase	(+)B
IDI1/2	Idi	Isoprenoid synthesis	Isopentenyl-diphosphate delta-isomerase	(−)B
IDH1	Idh	α-Oxidation	Isocitrate dehydrogenase	(−)B
LONP	Lon	Protease	Lon protease	(+)B
MARF1	Marf1	mRNA stability	Meiosis regulator and mRNA stability	(−)B
NUDT7	CG11095		Coenzyme A diphosphatase	(−)B
PAOX	CG8032		Spermine/spermidine oxidase	(−)B
PHYH	CG14688	α-Oxidation	Phytanoyl-CoA dioxygenase	(−)B
PMVK	CG10268	Isoprenoid synthesis	Phosphomevalonate kinase	(+)B
PRDX1	Jafrac	ROS metabolism	Peroxiredoxin	(−)B
PRDX5/PMP20	Prx5	ROS metabolism	Peroxiredoxin	(−)B
XDH	Ry	Purine metabolism	Xanthine dehydrogenase	(+)B
ACSF3	CG18155	β-Oxidation	Long-chain-fatty-acid–CoA ligase Malonate–CoA ligase	(+)B
NUDT19	CG10194		Coenzyme A diphosphatase	(+)B
MUL1	Mul1		Mitochondrial E3 ubiquitin protein ligase	(+)B
MVK	CG33671	Isoprenoid synthesis	Mevalonate kinase	(−)B
NOS2	Nos	ROS metabolism	Nitric oxide synthase	(+)B
SERHL/SEHL2	Kraken	Protease	Serine hydrolase-like	(−)B
SOD2	Sod2	ROS metabolism	Superoxide dismutase	(−)B

“Loc”: (+) experimentally validated localization to peroxisomes, (−) did not localize to peroxisomes in B-(Baron et al., 2016), F-(Faust et al., 2012), H-(Huang et al., 2016) or HT-(Hehlert et al., 2019).