Table 1.
Descriptive statistics of various assays of IOMM-Lee cells.
| Different assays | Number | Group (Mean ± Standard deviation) | |||
|---|---|---|---|---|---|
| Control | Scramble | Mimic | Inhibitor | ||
| Absorbance (450 nm) | |||||
| 0 Gy | 3 | 0.724 ± 0.008 | 0.705 ± 0.011 | 0.938 ± 0.032 | 0.450 ± 0.023 |
| 2 Gy | 3 | 0.649 ± 0.029 | 0.660 ± 0.023 | 0.883 ± 0.016 | 0.387 ± 0.006 |
| 4 Gy | 3 | 0.545 ± 0.007 | 0.555 ± 0.024 | 0.754 ± 0.020 | 0.307 ± 0.009 |
| 6 Gy | 3 | 0.383 ± 0.016 | 0.406 ± 0.022 | 0.612 ± 0.005 | 0.210 ± 0.008 |
| 8 Gy | 3 | 0.174 ± 0.021 | 0.183 ± 0.010 | 0.374 ± 0.011 | 0.108 ± 0.006 |
| Colony number | |||||
| 0 Gy | 3 | 454.333 ± 8.021 | 456.333 ± 8.021 | 487.667 ± 3.786 | 429.000 ± 2.000 |
| 2 Gy | 3 | 338.333 ± 9.713 | 334.000 ± 4.583 | 393.000 ± 10.536 | 269.000 ± 3.606 |
| 4 Gy | 3 | 238.000 ± 3.000 | 221.000 ± 5.568 | 323.333 ± 5.686 | 160.333 ± 2.082 |
| 6 Gy‡ | 3 | 150.000 ± 3.606 | 136.667 ± 2.517 | 195.000 ± 5.000 | 114.000 ± 3.000 |
| 8 Gy‡ | 3 | 129.667 ± 4.933 | 123.333 ± 4.163 | 169.667 ± 3.055 | 55.667 ± 1.528 |
| Apoptotic percentage (%) | |||||
| 0 Gy | 3 | 7.850 ± 0.684 | 6.897 ± 0.302 | 2.407 ± 0.057 | 27.473 ± 1.033 |
| 2 Gy | 3 | 9.587 ± 0.023 | 10.190 ± 0.710 | 3.313 ± 0.201 | 29.540 ± 0.376 |
| 4 Gy | 3 | 16.490 ± 0.154 | 15.607 ± 0.580 | 3.457 ± 0.068 | 33.413 ± 1.196 |
| 6 Gy | 3 | 19.743 ± 0.321 | 17.750 ± 0.700 | 4.340 ± 0.147 | 35.213 ± 0.671 |
| 8 Gy | 3 | 23.503 ± 0.588 | 24.743 ± 0.337 | 4.570 ± 0.101 | 35.717 ± 0.611 |
| Cell cycle distribution (%)¶ | |||||
| G0/G1 phase | |||||
| 0 Gy | 3 | 38.412 ± 2.400 | 43.192 ± 0.614 | 34.748 ± 1.102 | 48.659 ± 0.316 |
| 2 Gy | 3 | 42.388 ± 1.074 | 47.115 ± 1.426 | 41.311 ± 2.411 | 48.919 ± 1.052 |
| 4 Gy | 3 | 46.981 ± 2.555 | 49.741 ± 0.699 | 41.694 ± 1.472 | 50.139 ± 1.066 |
| 6 Gy | 3 | 47.949 ± 0.743 | 52.801 ± 3.184 | 43.921 ± 0.920 | 55.094 ± 2.479 |
| 8 Gy | 3 | 51.974 ± 0.791 | 53.391 ± 1.913 | 47.220 ± 0.725 | 57.241 ± 3.615 |
| S phase | |||||
| 0 Gy | 3 | 46.262 ± 6.932 | 36.459 ± 0.114 | 39.013 ± 5.147 | 36.517 ± 3.209 |
| 2 Gy | 3 | 27.145 ± 3.136 | 40.027 ± 0.962 | 36.787 ± 8.346 | 35.985 ± 9.891 |
| 4 Gy | 3 | 32.658 ± 5.989 | 24.266 ± 4.618 | 33.796 ± 5.214 | 43.053 ± 3.468 |
| 6 Gy | 3 | 42.525 ± 4.232 | 36.462 ± 4.193 | 32.500 ± 5.032 | 36.545 ± 8.268 |
| 8 Gy | 3 | 30.511 ± 2.680 | 32.301 ± 6.639 | 31.983 ± 4.564 | 28.289 ± 4.606 |
| G2/M phase | |||||
| 0 Gy | 3 | 15.326 ± 8.769 | 21.153 ± 0.962 | 14.006 ± 7.538 | 15.534 ± 3.943 |
| 2 Gy | 3 | 20.881 ± 2.394 | 16.782 ± 1.038 | 16.099 ± 9.762 | 14.274 ± 10.200 |
| 4 Gy | 3 | 14.540 ± 9.162 | 22.343 ± 6.500 | 31.456 ± 6.072 | 15.637 ± 5.816 |
| 6 Gy | 3 | 15.781 ± 5.692 | 19.617 ± 4.586 | 20.280 ± 5.735 | 14.796 ± 8.574 |
| 8 Gy | 3 | 20.570 ± 2.853 | 17.560 ± 7.330 | 12.924 ± 6.696 | 14.470 ± 8.155 |
| Invasive cell number | |||||
| 0 Gy | 5 | 47.800 ± 1.643 | 52.600 ± 4.980 | 77.000 ± 4.583 | 37.800 ± 5.215 |
| 2 Gy | 5 | 61.600 ± 4.827 | 62.000 ± 6.403 | 87.200 ± 9.039 | 47.000 ± 7.517 |
| 4 Gy | 5 | 68.200 ± 3.194 | 70.200 ± 4.658 | 132.400 ± 20.959 | 47.800 ± 1.304 |
| 6 Gy | 5 | 100.400 ± 19.424 | 91.600 ± 13.334 | 178.200 ± 25.024 | 60.400 ± 7.893 |
| 8 Gy | 5 | 186.200 ± 47.108 | 167.600 ± 48.993 | 256.000 ± 25.318 | 81.400 ± 5.320 |
‡
These results were normalized as the colony numbers per 1000 seeded cells.
¶
These results, after the exclusion of “< 2N” and “> 4N” parts, were normalized by using the geometric proportion method to achieve the sum of persentages of G0/G1, S, G2/M phases of each subgroup is 100%.