Table 3.
Differentially culturable tubercle bacilli (DCTB) assay results comparing growth in culture filtrate supplemented media (CFSM) and media only, when using three sample types [bronchoalveolar lavage fluid (BALF), post-bronchoscopy (PB) sputum and induced sputum] in 22 EOT patients.
| PID | BALF | PB sputum | Induced sputum | ZN stain | BAP | Selecta | Recovered strain type | |||
|---|---|---|---|---|---|---|---|---|---|---|
| CFSM | Media | CFSM | Media | CFSM | Media | |||||
| 250 | - | - | 3/3 | 1/3 | 2/3 | 1/3 | NEG | POS | NEG | NA |
| 253 | 1/3 | - | 2/3 | 3/3 | 3/3 | 1/3 | POS | NEG | NEG | 1 BEIJING |
| 267 | 3/3 | - | 3/3 | 3/3 | 3/3 | 1/3 | POS | NEG | POS | 373 T1 |
| 273 | 1/3 | 1/3 | NEG | NA | NA | NA | ||||
| 278 | - | - | - | - | - | - | NA | POS | NEG | NA |
| 291 | * | * | 1/3 | 1/3 | 3/3 | 1/3 | NEG | POS | NEG | NA |
| 300 | 1/3 | - | 2/3 | 1/3 | 3/3 | 1/3 | NEG | POS | NEG | NA |
| 306 | - | - | 1/3 | - | 3/3 | 2/3 | NEG | POS | NEG | NA |
| 314 | 1/3 | - | - | 1/3 | - | - | NEG | POS | NEG | NA |
| 315 | 1/3 | 1/3 | 1/3 | 1/3 | 3/3 | 3/3 | NEG | POS | NEG | NA |
| 318 | 1/3 | 1/3 | 1/3 | 2/3 | 2/3 | NEG | POS | NEG | NA | |
| 334 | - | - | - | - | 3/3 | 2/3 | NEG | POS | NEG | NA |
| 332 | - | - | - | - | - | - | NA | NA | NA | NA |
| 335 | 1/3 | 1/3 | 1/3 | 1/3 | 1/3 | 1/3 | NEG | POS | NEG | NA |
| 338 | 3/3 | 1/3 | 2/3 | 3/3 | 1/3 | 2/3 | POS | NEG | POS | 1 BEIJING |
| 351 | - | - | - | - | 1/3 | 2/3 | NEG | POS | NEG | NA |
| 357 | - | - | - | - | - | - | NA | NA | NA | NA |
| 359 | - | - | 1/3 | - | 1/3 | - | NEG | POS | NEG | NA |
| 365 | 1/3 | - | - | 1/3 | 1/3 | 1/3 | NEG | POS | NEG | NA |
| 375 | - | - | - | - | 1/3 | 2/3 | NEG | POS | NEG | NA |
| 382 | - | 1/3 | 2/3 | 1/3 | 1/3 | 1/3 | NEG | POS | NEG | NA |
| 392 | - | - | 1/3 | - | 1/3 | - | NEG | POS | NEG | NA |
| % Positive | 41 | 22.7 | 54.5 | 54.5 | 81.8 | 72.7 | ||||
| % True positive | 13.6 | 4.5 | 4.5 | 0 | 9 | 0 | ||||
Recovered Mtb strain type was determined using spoligotyping. The true positives row indicates how many of the positive cultures were confirmed to be Mtb using confirmatory assays.
Growth is indicated as either positive in three of the replicate wells (3/3), two of the three replicates (2/3) or one of the three replicate wells (1/3) or no growth (-). Gray blocks indicate that a sample was not available.
Indicates the wells became contaminated with fungal growth and confirmatory assays could not be conducted. The red blocks indicate the samples that were confirmed positive for Mtb.