Figure 2.

Gene knockout of EGFL6 by CRISPR/Cas9 and identification of SKOV3 cells. The sgRNA was designed according to exon 2 of the human EGFL6 gene. The CRISPR/Cas9 lentivirus was packaged in 293T cells, SKOV3 cells were infected with CRISPR/Cas9 lentivirus, cultured using puromycin, and the monoclonal cell lines were screened using the limited dilution method. (A) Construction of sgRNA into lentiCRISPRv2 plasmid. (B) Western blot for EGFL6 in E10 and G11, validating the loss of protein expression. (C) Sequences of wild-type SKOV3 (WT), negative control group (NC), and the selected clones E10 and G11, validating the deletion of base pairs in exon 2.