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. 2020 Aug 25;11:1903. doi: 10.3389/fimmu.2020.01903

Figure 2.

Figure 2

TGF-β does not enhance hypoxia-induced VEGF-A production. (A) VEGFA gene expression relative to daily normoxic basal media after 3 days in culture with or without TGF-β (10 ng/mL) or hypoxia (1% O2). (B) Secreted VEGF-A per 100,000 live cells after 3 days in culture with or without TGF-β (10 ng/mL) and hypoxia (1% O2). (C) Absolute secreted VEGF-A after 3 or 7 days with or without TGF-β (10 ng/mL) and hypoxia (1% O2). (D) Schematic depiction of the culture conditions in (C). After 3 days in hypoxia, a subset of NK cells were returned to normoxia for 4 days to observe whether VEGF-A production persists when cells re-enter the normoxic state. **p < 0.01, ***p < 0.001, ****p < 0.0001 by two-way ANOVA with Sidak's post-hoc test to determine effects of TGF-β. #Indicates measurement below detection limit of ELISA.