Figure 6.
Effect of 18 on phosphorylation of HER2 from lung tumor tissue sections of mice harvested at 35th day. Frozen tumors were processed and Western blot was carried out. (Blots were cropped and presented). Quantification of signal intensities were done by ImageJ). A) Western blot images of HER-2, p-HER-2 and GAPDH. B) Quantitative analysis of Western blot images indicating reduction in phosphorylation of p-HER2 by 18 and lapatinib (10 mg/kg) compared to the control. C) Inhibition of EGFR dimerization in NSCLC tissues from mice by 18 (6 mg/kg) using PLA. Representative tissue sections from lungs of mice from different group of animals were subjected to PLA. Red fluorescence indicates dimerization of EGFR:HER2 (top panel) and HER2:HER3(bottom panel). Expanded regions are shown as insets. Note the reduction in the number of red fluorescence dots in 18-treated lung tissue. Magnification 40×, size of scale bar 20 μm. Nuclei were stained with DAPI.