MAPK11 reversed the effect of TRIM67 on cell proliferation, colony formation, migration, and invasion in SW480 cells. TRIM67 and MAPK11 were knocked down in SW480 cells. A Co-depletion of TRIM67 and/or MAPK11 was detected by RT-qPCR. B Effects on SW480 cell viability were detected by CCK-8 assay. C Effects on SW480 colony formation were detected by colony formation assay. D Effects on SW480 cell migration were detected by cell migration assay. E Effects on SW480 cell invasion were detected by invasion assay. Results show mean ± SD from three independent experiments. ****P<0.0001, ***P<0.001, **P<0.01, *P<0.0.