Figure 2.
Effects of TGF-β1 on L929 cell proliferation after transfection with 200 ng of c-Ski-RNAi plasmid. A. c-Ski knockdown significantly inhibited the proliferative effects of different concentrations of TGF-β1 (n=12); Student's t-test, *P<0.05, **P<0.01 vs. the corresponding dose of control RNAi. B. Effects of different doses of the c-Ski-RNAi plasmid on proliferation (n=12); Student's t-test, *P<0.05, **P<0.01 vs. the adjacent dose. C. Changes in c-Ski mRNA expression after injection of different doses of the c-Ski-RNAi plasmid (n=9); Student's t-test, *P<0.05, **P<0.01 vs. the adjacent dose. Changes in c-Ski protein expression following TGF-β1 treatment for 4 h (D) and 24 h (E) after injection of 200 ng of c-Ski RNAi (n=3) in three independent experiments; Student's t-test, **P<0.01 vs. the control in each group; #P<0.05, ##P<0.01 vs. control RNAi.
