Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 7;10:14700. doi: 10.1038/s41598-020-71626-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Relationship in fundus autofluorescent spots, apoptosis of photoreceptors and retinal ONL thickness in the central retina of rd10 raised in dark. (A) Pictures show the time course change from P16 to P60, in the central retina of rd10 mice raised in dark. Left pictures are in vivo FAF imaging with a retinal imaging microscope. Middle pictures show the confocal images of extracted flat-mounted retina at the ONL level, labeled with ssDNA (white). Scale bars, 100 μm, “ + ” show the center of the optic nerve head. Right pictures show the retinal section labeled with DAPI (blue), ssDNA (white) and Iba1 (red). Scale bars, 50 μm. (B) The graphs show the quantification of the FAF spot area % (green line), the ssDNA⁺ area % (black line), the thickness of the ONL (blue line) and the thinning rate of the ONL (magenta line). The markers of the graph show mean and SD (n = 4). (†p < 0.005, ††p < 0.00005, †††p < 0.00000005, t test in comparison to the central retina raised under regular light cycle). Thinning of ONL was significantly suppressed compared to the group raised under regular light cycle. The appearance of FAF spots and ssDNA⁺ apoptotic cells were also suppressed in dark-raised group.