Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 7;11:4435. doi: 10.1038/s41467-020-18288-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Isolation of cycling G2/M cells by flow cytometry. Cells were stained with the live DNA stain Dye Cycle Ruby. Linear analysis of fluorescence intensity plotted against forward scatter (cell size) shows a clear separation of G2/M cells with 4n DNA content and G0/G1 cells with 2n DNA content. G2/M cells comprise 10% of live cells. b qPCR analysis showing expression of, integrin-alpha-6, cyclin b, pou3, notch1, notch2, hes1, frizzled 5/8, disheveled (dshv), and beta catenin in G2/M cells in steady state (0 h). Data are expressed as fold changes normalized to G0 cells from three independent experiments. Box plot shows interquartile range (the distance between the upper and lower quartiles) and whiskers indicate the minimum and maximum of the data. c qPCR analysis showing expression of cyclin b, integrin-alpha-6, pou3, notch1, notch2, and frizzled 5/8 in G2/M cells at 24 and 48 h post injury. Data are expressed as fold changes normalized to actin, from three independent experiments. Box plot shows interquartile range (the distance between the upper and lower quartiles) and whiskers indicate the minimum and maximum of the data. Source data are provided as a Source Data file.