Table 6. Transcript numbers in E19 placenta and fetal brain for negative acute phase plasma proteins and IL1β.
| E19 placenta | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Control | Acute | Chronic | ||||||||||
| Sample | Afp | Tf | Ttr | IL1β | Afp | Tf | Ttr | IL1β | Afp | Tf | Ttr | IL1β |
| 1 | 10372 | 2906 | 783 | 2.7 | 3.5 | 31 | 0.7 | 1.3 | 2.7 | 35 | 1.4 | 11 |
| 2 | 16873 | 3789 | 1100 | 2.5 | 3.0 | 35 | 2.6 | 2.2 | 3.5 | 28 | 1.6 | 30 |
| 3 | 1263 | 366 | 65 | 2.3 | 1571 | 413 | 95 | 3.3 | 3.5 | 17 | 1.7 | 46 |
| 4 | 72 | 56 | 4.6 | 3.8 | 8.4 | 29 | 1.6 | 2.2 | 2.9 | 45 | 1.1 | 58 |
| E19 brain | ||||||||||||
| Control | Acute | Chronic | ||||||||||
| Sample | Afp | Tf | Ttr | IL1β | Afp | Tf | Ttr | IL1β | Afp | Tf | Ttr | IL1β |
| 1 | 0.4 | 2.8 | 0.2 | 0.1 | 0.2 | 1.2 | 2.3 | 0.1 | 0.2 | 2.9 | 0.8 | 0.2 |
| 2 | 1.7 | 2.1 | 0.5 | 0.1 | 0.1 | 1.2 | 0.4 | 0.1 | 0.1 | 3.2 | 1.1 | 0.1 |
| 3 | 0.2 | 5.0 | 0.4 | 0.2 | 0.1 | 1.9 | 0.2 | 0.1 | 0.1 | 3.5 | 0.9 | 0.1 |
| 4 | 0.1 | 3.3 | 0.2 | 0.1 | 0.2 | 1.4 | 0.3 | 0.1 | 0.2 | 2.8 | 1.0 | 0.1 |
In placental samples transcript counts per million for the three negative acute phase proteins were smaller in chronically treated animals compared to controls and all IL1β numbers were greater than in controls. There was some variation in values between individual placentas which was more obvious in samples from acutely treated animal indicating that the response was potentially time-dependent. In all brain samples the transcript numbers were very low, with no evidence of an inflammatory response. This indicates that the response in the placenta was tissue specific.