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. 2020 Aug 31;142(10):100805. doi: 10.1115/1.4048028

Fig. 10.

Pathlines corresponding to the trajectory of fluorescent particle for 22.5 s in the lymphatic vessels of the (a) normal and (b) affected mice. The pathlines were used to generate representative flow condition waveforms. LECs were exposed in vitro to (c) pulsatile ML (+ + +) or static lymphatic sinus (○ ○ ○) representative flow conditions. In vitro LEC (d) ICAM-1, ICAM-2, JAM-A, LYVE-1 and (e) CCL2, CXCL2, and IL8 mRNA expression of static lymphatic sinus condition normalized by pulsatile ML waveform (n = 7, ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001). In vitro LEC (f) ICAM-1, ICAM-2, JAM-A, LYVE-1 protein expression of static lymphatic VS condition normalized by pulsatile ML waveform (n ≥ 4, ANOVA, *P < 0.05, **P < 0.01).

Pathlines corresponding to the trajectory of fluorescent particle for 22.5 s in the lymphatic vessels of the (a) normal and (b) affected mice. The pathlines were used to generate representative flow condition waveforms. LECs were exposed in vitro to (c) pulsatile ML (+ + +) or static lymphatic sinus (○ ○ ○) representative flow conditions. In vitro LEC (d) ICAM-1, ICAM-2, JAM-A, LYVE-1 and (e) CCL2, CXCL2, and IL8 mRNA expression of static lymphatic sinus condition normalized by pulsatile ML waveform (n = 7, ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001). In vitro LEC (f) ICAM-1, ICAM-2, JAM-A, LYVE-1 protein expression of static lymphatic VS condition normalized by pulsatile ML waveform (n ≥ 4, ANOVA, *P < 0.05, **P < 0.01).