Table 1.
Primers for quantitative polymerase chain reaction mRNA analysis
| Gene | Locus ID | Forward primer (5′–3′) | Reverse primer (5′–3′) | Fragment (bp) |
|---|---|---|---|---|
| ICAM-1 | NM_000201.1 | GCGTAGGGTAAGGTTCTTGC | GCTATTCAAACTGCCCTGATG | 128 |
| ICAM-2 | NM_000873.5 | ACTCAATGGTGAAGGACTTGC | TCCAATGCCACTTCACCTG | 135 |
| JAM-A | NM_016946.1 | TGACTTCAGGTTCAGAAGAGTG | GCAAGTCGAGAGGAAACTGT | 101 |
| LYVE-1 | NM_006691.1 | CCATAATTCTGCATGACACCTG | ATCTGGACCACGAGGCT | 79 |
| CCL2 | NM_002982.1 | GCCTCTGCACTGAGATCTTC | AGCAGCCACCTTCATTCC | 104 |
| CXCL2 | NM_002089.4 | CATCGAAAAGATGCTGAAAAATG | TTCAGGAACAGCCACCAATA | 77 |
| IL-8 | NM_000584.1 | CTTCACACAGAGCTGCAGAA | GAGACAGCAGAGCACACAAG | 143 |
| GAPDH | NM_002046.1 | TGTAGTTGAGGTCAATGAAGGG | ACATCGCTCAGACACCATG | 143 |