Table 2.
Primary and secondary antibodies for Western Blot analysis
| Antibody | Host species | Vendor | Catalog # | Working dilution |
|---|---|---|---|---|
| ICAM-1 | Rat | Santa Cruz Biotechnology, Dallas, TX | sc-52553 | 1:500 |
| ICAM-2 | Mouse | Santa Cruz Biotechnology, Dallas, TX | sc-9987 | 1:500 |
| JAM-A | Rabbit | Thermo Fisher Scientific, Waltham, MA | 36-1700 | 1:250 |
| LYVE-1 | Rat | R&D Biosystems, Minneapolis, MN | MAB2125 | 1:500 |
| β-Tubulin | Mouse | Santa Cruz Biotechnology, Dallas, TX | 166729 | 1:1000 |
| Goat IgG HRP | Rabbit | Invitrogen, Carlsbad, CA | 81-1620 | 1:1000 |
| Mouse IgG HRP | Goat | Thermo Fisher Scientific, Waltham, MA | 31430 | 1:1000 |
| Rabbit IgG HRP | Goat | Thermo Fisher Scientific, Waltham, MA | 31460 | 1:1000 |
| Rat IgG HRP | Goat | Thermo Fisher Scientific, Waltham, MA | 31470 | 1:1000 |