Breast cancer cell migration speed is dependent on metastatic potential, shear stimulation, and time after shear. (a) MCF-10A exhibited low response to flow stimulation with only an increase in cell speed at shear onset. (b) and (c) The MDA-MB-231 line may exhibit characteristics related to increased metastasis by migrating significantly faster than MDA-MB-468. The greatest speed of the MDA-MB-231 line was seen at shear onset with the speed decreasing until reaching a rather constant value after about 30 min. #: comparison with MCF-10A static. *:comparison with MDA-MB-468 static. ‡: comparison with MDA-MB-468 FF15. +: comparison with MDA-MB-231 static. Other comparisons did not reach statistical significance. Single, double, and triple symbols represent p < 0.05, p < 0.01, and p < 0.001, respectively.