Superoxide anion production and PKC activity were assessed in PMNs incubated for 45 min at 37° C with PBS or fMLP (1 μM) (A and B). PMNs were pre-incubated with PBS or fMLP (1 μM) then incubated for 4 h with HDLs (1 mg protein/ml). HDL-associated miR-223 levels pre- and post-incubation (C), intracellular mature miR-223–3p levels (D), pri-miR-223 levels (E), and Dicer mRNA levels (F) were quantified by real-time PCR with relative quantitative values reported as fold-change (N≥3). Data are presented as mean ±SEM (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).