KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-pY490-TRKA | Cell Signaling | Cat# 9141S; RRID: AB_2298805 |
| Anti-HA 6E2/2367 | Cell Signaling | Cat# C29F4/3324S; RRID: AB_1549585 |
| Anti-total TRK A7H6R | Cell Signaling | Cat# 92991S; RRID: AB_2800196 |
| Anti-Cytokeratin 19 D4G2 XP/12434 | Cell Signaling | Cat# D7F7W/13092S; RRID: AB_2722626 |
| Anti-β-Tubulin 9F3/2128 | Cell Signaling | Cat# D3U1W/86298S; RRID: AB_2715541 |
| Anti- pT202/pY204-ERK1/2 D.13.14.4E | Cell Signaling | Cat# 437OS; RRID: AB_10694057 |
| Anti- total ERK L34F12 | Cell Signaling | Cat# 4696S; RRID: AB_10694988 |
| Anti-BIM Y36 | AbCam | Cat# ab32158; RRID: AB_725697 |
| Anti-PARP | Cell Signaling | Cat# 9542S; RRID: AB_2160739 |
| Anti-PUMA | Cell Signaling | Cat# 4976S; RRID: AB_2064551 |
| Anti-BAX | Cell Signaling | Cat# 2772S; RRID: AB_10695870 |
| Anti-pY1068/pY1173-EGFR 1H12/2236S | Cell Signaling | Cat# 53A5/4407S; RRID: AB_331795 |
| Anti-total EGFR D38B1 | Cell Signaling | Cat# 4267S; RRID: AB_2246311 |
| Anti-pS473-AKT D9E | Cell Signaling | Cat #4060L; RRID: AB_2315049 |
| Anti-total AKT 40D4 | Cell Signaling | Cat# 2920S; RRID: AB_1147620 |
| Anti-BMF G81 | Cell Signaling | Cat# 5889; RRID: AB_10835694 |
| Anti-Actin 8H10D10 | Cell Signaling | Cat# 3700; RRID: AB_2242334 |
| Anti-GAPDH D16H11 | Cell Signaling | Cat# 5174; RRID: AB_10622025 |
| anti-pTyr | Millipore | Cat# 4G10; RRID: AB_916370 |
| Anti-Phospho BIM S69 | McMahon Lab | PMID:18715233 |
| Bacterial and Virus Strains | ||
| Stbl3 Chemically Competent E.coli | Thermo Fisher | C73733 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Entrectinib | Ignyta/Genentech | MTA |
| Cobimetinib | Genentech | MTA |
| Gefitinib | SelleckChem | S1025 |
| IFN Gamma | Peprotech | 315–05 |
| IL-3 | Peprotech | 213–13 |
| D-Luciferin | Gold Bio | LUCK-100 |
| Critical Commercial Assays | ||
| Cell Titer 96 Acqueous One | Promega | G3582 |
| Proteome Profiler Mouse RTK array | R&D | ARY014 |
| Experimental Models: Cell Lines | ||
| IMPE (mouse) | University of Rochester | PMID:18500333 |
| 293-T (human) | ATCC | CRL-3216 |
| MLE-12 (mouse) | ATCC | CRL-2110 |
| Ba/F3 (mouse) | ATCC | N/A currently |
| CUTO-3.29 (human) | University of Colorado (MTA) | PMID: 24162815 |
| KM12 (human) | NCI (MTA) | PMID: 24162815 |
| Experimental Models: Organisms/Strains | ||
| Mouse: NOD.Cg-Prkdcscld/J | HCI PRR Colony | internal colony |
| Oligonucleotides | ||
| 5′-GCACAGGAGCTGCGGCGGAT-3′ (sgBIM) | IDT | This study |
| Recombinant DNA | ||
| TPR-NTRK1-pLenti-6 | Genscript | This study |
| pCMV-VSV-G | Addgene | 8454 |
| psPAX2 | Addgene | 12260 |
| pFUGW-Pol2-ffLuc2-eGFP | Addgene | 71394 |
| EGFR WT-pBABE | Addgene | 11011 |
| EGFR-L858R-pBABE | Addgene | 11012 |
| pLenti-CRISPRv2 | Addgene | 52961 |