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. 2020 Jul 2;127(7):928–944. doi: 10.1161/CIRCRESAHA.119.315528

Figure 5.

Figure 5.

Heme-regulated gene expression in mBMM and effects of AMPK (AMP-activated protein kinase) or ATF1 (activating transcription factor-1) deficiency. A, mBMMs from Prkab1−/−, Atf1−/−, or WT (wild type) littermate controls were stimulated in vitro by 10 μM heme and cells harvested after varying durations for assay of mRNA transcripts by reverse transcription-quantitative polymerase chain reaction, as in Figure 3B. Data points are mean±SE, n=6; Exact P given for repeated measures ANOVA. B, Summary of the impact of AMPK and ATF1 deficiency, which had very similar effects. Abca1 indicates ATP-binding cassette family subfamily a member 1; ApoE, apolipoprotein E; HO-1, heme oxygenase-1; Igf1, insulin-like growth factor-1; Lxr, lipid X receptor; Pdgf, platelet derived growth factor; Prkab1, protein kinase AMP-activated beta subunit 1; and Socs1, silencer of cytokine signaling 1.