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. 2020 Sep 1;33(7):494–506. doi: 10.1089/vim.2019.0188

FIG. 1.

FIG. 1.

CVB4 abundance and location in the kidney over time. (A) RT-qPCR of CVB4 RNA normalized by the geometric mean of Gapdh and Actg1 (n = 4–8; *p < 0.05 compared with day 0, @p < 0.05 compared with day 2, &p < 0.05 compared with day 3). (B) Representative images from ISH analysis of +ssCVB4 RNA (genomic) 2 days (upper two panels) and 14 days (lower two panels) postinoculation. CVB4 RNA was detected in the glomerular (left) and tubulointerstitial (right) regions of the kidneys of inoculated mice 2 days postinoculation; however, 14 days postinoculation, CVB4 was only found in the glomerular region. Arrowheads point to labeled CVB4 RNA (representative images taken at 400 × magnification; n = 4). Actg1, gamma-actin; CVB4, coxsackievirus B4; Gapdh, glyceraldehyde 3-phsophate dehydrogenase; ISH, in situ hybridization; RT-qPCR, reverse transcription real-time polymerase chain reaction.