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Viral PRR expression and downstream targets. RT-qPCR of (A) Ifnb, (B) Tnfa, (D) Irf3, (B) Irf1, (C) Irf5, and (D) Irf7 0, 2, 3, 5, 7, 9, 10, and 14 days post-CVB4 inoculation, normalized to the geometric mean of Gapdh and Actg1. Only seven of eight kidneys at 3 days and one of four kidneys at 5 days postinoculation had detectable levels of Ifnb (n = 4–8; *p < 0.05 compared with day 0, @p < 0.05 compared with day 2, &p < 0.05 compared with day 3). PRR, pattern recognition receptor.
