Figure 7. Decreased Staining for Lysosomal but Not SV Proteins at Diaphragm Neuromuscular Junctions and Evidence of Neuroaxonal Dystrophy in Myrlysin-KO Embryos.

(A) Whole diaphragms were harvested from WT and myrlysin-KO E18 embryos and co-stained with antibodies to LAMP1 and SV2 and with Alexa Fluor 594-conjugated α-bungarotoxin (BTX). Single-channel images are shown in inverted grayscale. Arrowheads point to individual NMJs. Scale bars: 20 μm. Notice the absence of LAMP1 staining in KO NMJs. See also Figure S6.

(B) H&E staining of sagittal sections from whole WT and KO E18 embryos. The dashed lines demarcate the boundaries of the phrenic and spinal nerves. Arrowheads indicate dystrophic eosinophilic bodies corresponding to swollen axons. Scale bars: 200 μm.

Insets in (A) and (B) are magnified views of the boxed areas.