Figure 1.
Confirmation of gp100 peptide conjugation to Maleimide-PEG2000-DSPE via (A) TLC and (B) HPLC methods
(A) The final product of linking reaction was spotted on silica thin-layer plates besides the free peptide and Maleimide-PEG2000-DSPE, for chromatography in Chloroform: Methanol: Water (45:9:1) mobile phase. (B) Analysis of the final product by High-pressure Liquid Chromatography (HPLC) using Nucleosil C18 column with elution of ACN: Water (70:30) containing 0.1% TFA at 220 nm. The percentage of conjugation was determined according to the extent of unconjugated peptide (with retention of ~ 8 mins)