Figure 2.

Flow cytometric analysis of BMDCs maturation

Immature bone marrow-derived dendritic cells (day 7) were stimulated with gp100 peptide (2.5 μg/ml) and cationic liposome (12 nmol/ml) for 8 hours, the same time used for LPS (1µg/ml). Afterward, DCs were stained for DCs specific marker, CD11c, and then for co-stimulatory molecules CD40, CD80, CD86 and MHC II molecule. For the analysis, DCs were gated for CD11c⁺ cells followed by gating for the selected markers. Immature DCs were analyzed on day 5. The numbers in the dot plot graph represent the percentages of matured DCs