Figure 5.

Therapeutic effects of liposomal nanovaccine in combination with anti PD-1 mAb

Immune responses followed combination therapy in TME. (S5A) Representative dot plots demonstrating the gating strategies for flow cytometric analysis of CD₃⁺ T cells and regulatory TILs. (B) Frequency of CD₃⁺ cells indicating TILs, (C) Frequency of CD₈ in CD₃⁺ gated TILs, (D) Frequency of PD-1 in CD₈⁺ TILs, (E) Frequency of IFN-γ in CD₈⁺ TILs. (F) Frequency of CD₄ in CD₃⁺ gated TILs, (G) Frequency of PD-1 in CD₄⁺ TILs, (H) Frequency of IFN-γ in CD₄⁺ TILs, (I) Frequency of IL-4 in CD₄⁺ TILs. (J) Frequency of CD₄/CD₂₅/Foxp₃⁺ TILs, (K) Frequency of IL-10 in Foxp₃⁺ gated TILs. Results represented as Mean±SD; N=3. * P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001 indicated as statistically significant differences