Figure 5.

β-Arrestin-2 mRNA levels on hepatocytes after different doses of magnesium supplement treatment. RNA was isolated from rat liver tissue and reverse transcribed into DNA. Then PCR was performed using primers specific for β-Arrestin-2 and house keeping GAPDH gene. Gel electrophoresis was performed to compare the PCR products from each group. (A) Quantitative analysis of β-Arrestin-2 relative fold induction levels. (B) Representative picture of gel electrophoresis result. NC, normal control. DM, diabetic control. Mg, magnesium supplement. *P<0.05