Figure 3.
Ca2+ handling in Pluricyte® CMs. (A) Voltage-dependent ICaL activation; representative family of traces. (B) ICaL-density I–V relationship (n = 14). (C) Kinetics of inactivation of ICaL (single exponential fit). (D) Steady-state activation and inactivation, fitted by Boltzmann equation. The inset depicts window ICaL. (E) Simultaneous recordings of ICaL and Ca2+-transients (CaT) as a representative example. (F) Effect of caffeine (10 mmol/L) on membrane current and CaT, in a Pluricyte® CM. (G) Average values of CaT amplitude (above) and decay time constant (below), at baseline and during caffeine perfusion.